Table 3.
Molecular characterization of the 24 aEPEC mcr-1-positive isolates.
| Serotypea-PG-ST | CHb | No. isolates | Intimin typec (No. isolates) | Resistance profiled (isolates with mcr co-occurrence and type) |
|---|---|---|---|---|
| O2:H40-A-ST10 | 11-24 | 4 | eae-γ2 (3) | AMP AMP/SAM COL TI |
| AMP AMP/SAM CHL COL MI TI TMP/SMX | ||||
| AMP AMP/SAM CHL COL GEN TI TMP/SMX TOB | ||||
| eae-Θ2 (1) | CHL COL GEN TMP/SMX TOB | |||
| O26:H11-A-ST48 | 11-54 | 3 | eae-𝜀1 (3) | AMP AMP/SAM CHL COL TI |
| CHL COL GEN NAL TMP/SMX TOB (mcr-1/mcr-5) | ||||
| AMP AMP/SAM COL NAL TI TMP/SMX | ||||
| O157:H2-A-ST301 | 27-54 | 2 | eae-ξ (2) ∗ | AMP AMP/SAM CHL COL MI NAL TI TMP/SMX |
| AMP AMP/SAM CHL CIP COL GEN MI NAL TI TOB | ||||
| O45:H2-A-ST301 | 27-54 | 1 | eae-ξ (1) | AMP AMP/SAM COL TI |
| O157:H2-A-STNew1 | 27-54 | 1 | eae-ξ (1) ∗ | CHL COL GEN MI NAL TMP/SMX TOB |
| O26:H11-A-ST7367 | 685-54 | 1 | eae-𝜀1 (1) | COL MI |
| O103:H2-B1-ST20 | 4-25 | 1 | eae-β1 (1) | AMP AMP/SAM COL TI TMP/SMX |
| O111:H9-B1-ST29 | 4-24 | 2 | eae-β1 (2) | AMP AMP/SAM CHL COL ∗FOS NAL TI TMP/SMX |
| CHL COL ∗FOS NAL TMP/SMX | ||||
| O118:H9-B1-ST29 | 4-24 | 1 | eae-β1 (1) | COL NAL TMP/SMX |
| O123:H11-B1-ST29 | 4-24 | 3 | eae-β1 (3) | AMP CHL COL NAL TI TMP/SMX |
| COL MI | ||||
| CHL COL NAL TMP/SMX | ||||
| O177:H11-B1-ST29 | 4-440 | 1 | eae-β1 (1) | AMP AMP/SAM CHL CIP COL GEN LEV MI NAL TI TMP/SMX TOB |
| O26:H11-B1-ST29 | 4-440 | 1 | eae-β1 (1) | AMP AMP/SAM CHL COL ∗FOS TI (mcr-1/mcr-5) |
| O51:H9-B1-ST29 | 4-24 | 1 | eae-β1 (1) | AMP AMP/SAM CHL COL TI TMP/SMX |
| O145:H28-E-ST1034 | 23-331 | 1 | eae-γ1 (1) | AMP AMP/SAM CHL COL GEN TI TMP/SMX TOB |
| O45:H9-E-ST302 | 84-305 | 1 | eae-β1 (1) | AMP AMP/SAM CHL COL GEN MI NAL TI TMP/SMX TOB |
aNon-motile isolates were designated as HNM. PG, phylogroup; ST, sequence type. bCH, clonotype (fumC-fimH alleles). c∗HlyA positive isolates. dAMP, ampicillin; AMP/SAM, ampicillin-sulbactam; CHL, chloramphenicol; CIP, ciprofloxacin; COL, colistin; FOS, fosfomycin; GEN, gentamicin; LEV, levofloxacin, MI, minocycline; NAL, nalidixic acid; TI, ticarcillin; TMP/SMX, trimethoprim-sulfamethoxazole; TOB, tobramycin. ∗Isolates with a MIC value for FOS = 64 (according to EUCAST, the cut-off point is 32 mg/L and higher values are considered resistant, while for CLSI it is 64).