Fig. 5.
Reversal potential (Erev) becomes more negative when the membrane potential (Vm) is held at a more negative voltage. Families of whole cell currents evoked in a retinal pigment epithelial (RPE) cell from a holding potential of −120 mV in the presence of 140 mM external Cl− (A) or 140 mM external SCN− (B). The voltage-clamp protocol and scale for both sets of current are shown at the bottom of A and the zero-current level is shown by the horizontal lines to the left of currents. The effective series resistance (Rs) after series resistance compensation was 4 MΩ. Current-voltage (I-V) plots of the instantaneous current (C) or steady-state current (D) obtained from the same data as that shown in A and B in the presence of Cl− (open circles) or SCN− (closed circles). Voltages in C and D were corrected for liquid junction potentials and the voltage drop across the series resistance.