Skip to main content
. 2018 Jun 27;315(4):C502–C515. doi: 10.1152/ajpcell.00137.2018

Fig. 2.

Fig. 2.

Elevated transgelin (TAGLN) in gestational diabetes mellitus (GDM)-exposed endothelial colony-forming cells (ECFCs) contributes to impaired function. A: a representative Western blot is shown using whole cell lysates from ECFCs harvested from uncomplicated controls (UC) and ECFCs from GDM pregnancies treated with either a nonspecific siRNA (siControl) or a TAGLN-specific siRNA (siTAGLN). Membranes were probed for TAGLN and vinculin (VINC). B: phase contrast images of ECFC network formation on Matrigel 10 h postplating following siControl or siTAGLN treatments are illustrated. The numbers of closed networks formed were quantified and graphed (n = 8 transfections; *P = 0.019). C: Transwell migration assays were performed with ECFCs transfected with siControl or siTAGLN. Photomicrographs depict migrated ECFCs stained with crystal violet. The numbers of migrating cells after 4 h were quantified (n = 10 transfections; *P = 0.028).