Fig. 4.

Increasing transgelin (TAGLN) in endothelial colony-forming cells (ECFCs) from uncomplicated (UC) pregnancies is sufficient to impair function. A: representative Western blot is shown using whole cell lysates collected from gestational diabetes mellitus (GDM)-exposed ECFCs and ECFCs from uncomplicated pregnancies transduced with either a control lentivirus (EV control) or a TAGLN-expressing lentivirus (TAGLN OE). Membranes were probed for TAGLN and vinculin (VINC). B: phase contrast images of ECFC network formation on Matrigel following transduction with either EV control or TAGLN OE lentivirus. The numbers of closed networks were quantified 10 h postplating (n = 9 transductions, *P = 0.047). C: Transwell migration assays were performed with ECFCs transduced with EV control or TAGLN OE lentivirus. Photomicrographs depict migrated ECFCs stained with crystal violet 4 h after plating. The numbers of migrating cells after 4 h were quantified (n = 11 transductions, *P = 0.015).