Fig. 5.

Increasing transgelin (TAGLN) in endothelial colony-forming cells (ECFCs) from uncomplicated pregnancies does not alter proliferation. A: representative dot plots are shown for flow cytometric assessment of mitosis using an antibody against phosphorylated histone H3. The percentage of mitotic cells was quantified using freshly stained ECFC samples transduced with either a control lentivirus (EV control) or a TAGLN-expressing lentivirus (TAGLN OE). Staining intensity of phosphorylated histone H3-Alexa 488 is plotted on the x-axis and forward scatter (FSC) values are plotted on the y-axis. B: the mean percentage (±SE) of mitotic cells, determined by positive staining for phosphorylated histone H3, was quantified in EV control and TAGLN OE ECFCs (n = 8, P = 0.20, paired t-test). C: representative dot plots depict ECFC cell cycle distribution following transduction with EV control or TAGLN OE lentivirus. ECFCs were treated with bromodeoxyuridine (BrDU) and stained with 7-AAD (x-axis) and anti-BrDU-Alexa 488 (y-axis). D: the mean percentage (±SE) of ECFCs in G₁, S, and G₂ phases was quantified in EV control and TAGLN OE ECFCs (n = 8; P = 0.87, two-way ANOVA).