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. 2018 Jun 27;315(4):C502–C515. doi: 10.1152/ajpcell.00137.2018

Fig. 7.

Fig. 7.

Myosin light chain (MLC) phosphorylation is increased in endothelial colony-forming cells (ECFCs) with elevated transgelin (TAGLN). A: ECFC whole cell lysates from uncomplicated (UC) or gestational diabetes mellitus (GDM) pregnancies were evaluated for MLC phosphorylation (pMLC) and total MLC (MLC) by Western blotting. Nitrocellulose membranes were probed with antibodies against p-MLC, MLC, and vinculin (VINC). Quantification in ImageJ confirmed increased levels of pMLC relative to MLC in samples from GDM pregnancies (UC: n = 4; GDM: n = 6; **P = 0.003). B: similar Western blotting studies were conducted using whole cell lysates from ECFCs transduced with either a control lentivirus (EV control) or a lentivirus containing TAGLN (TAGLN OE). Quantitation in ImageJ confirmed increased levels of pMLC relative to MLC in TAGLN OE samples (n = 7 transductions; **P = 0.002).