Table 1.

In vivo morpholino sequences purchased from Gene Tools, designed according to company guidelines targeted for Tim23 knockdown and list of primer sequences used for real-time PCR analysis

Gene	In Vivo Morpholino Sequence
Tim23	5′-TCT TCC GCC ACC TTC CAT GAG GTC-3′
Control	5′-CCT CTT ACC TCA GTT ACA ATT TAT A-3′

	Primer
Gene	Forward	Reverse
Ddit3 (CHOP)	5′-CAC CAC ACC TGA AAG CAG AA-3′	5′-AGG TGA AAG GCA GGG ACT CA-3′
ATF5	5′-TGG AGC GGG AGA TCC AGT A-3′	5′-GAC GCT GGA GAC AGA CGT ACA-3′
Hspa9 (mtHsp70)	5′-TGG CTA TTA CTG CGG GTT CT-3′	5′-CAT CTG CTC CAC CTC CTC T-3′
Hspd1 (Hsp60)	5′-CTG GGT GCA AGA GCC ATA TA-3′	5′-GAA AGG CTG CTT CTG AAC TCT-3′
Hspe1 (cpn10)	5′-GGA GTG CTG CTG CCG AAA CTG TA-3′	5′-TCA CAC TGA CAG GCT CAA TCT-3′
Lonp1 (LonP)	5′-CGA CTT GCA CAG CCC TAT GT-3′	5′-CGA ATG TTC CCG TAT GGT AGA T-3′
Timm23 (Tim23)	5′-CCCGAGGCAGATTTGAACTA-3′	5′-AAAGCCAGGGAGCCTAGAGTAT-3′
B-actin	5′-TGT GAC GTT ACA TCC GTA A-3′	5′-GCT AGG AGC CAG AGG AGT AA-3′
18S rRNA	5′-GTAACCCGTTGAACCCCATT-3′	5′-CCATCCAATCGGTAGTAGCG-3′

All primers are designed for Mus musculus. ATF5; activating transcription factor 5; CHOP; C/EBP homologous protein; cpn10, chaperonin 10; Hsp60, heat shock protein 60; LonP, lon peptidase; mtHSP70, mitochondrial heat shock protein 70; Tim23, translocase of the inner membrane channel subunit 23.