Fig. 3.

Glial Ca²⁺ responses driven by muscarine are primarily mediated through M3Rs. Representative glial Ca²⁺ responses evoked by muscarine in the presence of the M5R selective antagonist VU0488130 (A) or the M3R selective antagonist J104129 (B). Gray traces show the responses of individual glial cells and the averaged response of all glia within the ganglion is shown in the black trace. Quantification of the effects of VU0488130 or J104129 on the number (D) and peak response (E) of glial Ca²⁺ responses induced by muscarine. (n = 21–39 glia from 4 to 5 mice; one-way ANOVA with multiple comparisons; *P < 0.05; **P < 0.005; ***P = 0.0009; ****P < 0.0001). ΔF/F, change in fluorescence over time; J104129, (αR)-α-cyclopentyl-α-hydroxy-N-[1-(4-methyl-3-pentenyl)-4-piperidinyl]benzeneacetamide fumarate; M3R, muscarinic type 3 receptor; M5R, muscarinic type 5 receptor; TTX, tetrodotoxin; VU0488130, 5-[(3-acetylphenoxy)methyl]-N-methyl-N-[(1S)-1-pyridin-2-ylethyl]-1,2-oxazole-3-carboxamide.