Continuous activation of glial hM3Dq receptors in vivo decreases pellet production and pellet fluid content without increasing colonic transit time. Effects of 0.13 mg/ml CNO in drinking water on colonic transit time (A); endogenous pellet production (B); pellet dry weight (C) and pellet water content (D) from GFAP::hM3Dq mice and WT littermates. (n = 3–6 mice; Student’s t-test; *P < 0.05; **P < 0.02). CNO, clozapine-N-oxide; GFAP, glial fibrillary acidic protein; hM3Dq, modified human M3R; M3R, muscarinic type 3 receptor; WT, wild-type.