Fig. 1.

Adipose tissue sirtuin 3 (SIRT3) expression in obese mice. A: gene expression of Sirt3, peroxisome proliferator-activated receptor-γ coactivator 1α(Pgc1α) and adiponectin (Adipoq), was determined in visceral adipose tissue (VAT) obtained from genetically obese (db/db) and high-fat diet (HFD)-induced obese mice and their lean controls (db/+ mice and regular-chow diet [RCD]-fed mice, respectively; n = 5 per group). B: VAT mitochondrial DNA (mtDNA) contents were normalized to nuclear DNA (nucDNA) contents (n = 3–5 per group). C: Western blot analysis of SIRT3 in VAT (n = 4–5 per group). Densitometric analysis of SIRT3 protein normalized to tubulin is shown. *Value significantly different from control value (Student’s t-test, P < 0.05). Values are means ± SE.