Fig. 2.

Generation of adipocyte-specific mitochondrial Sirt3 knockout (AMiSKO) mice. AMiSKO mice were generated by using Adipoq-Cre transgenic mice and floxed-Sirt3 (flox/flox) mice. A: Western blot analysis of SIRT3 in VAT, subcutaneous adipose tissue (SAT), brown adipose tissue (BAT), liver, skeletal muscle, and heart. B: gene expression of mitochondrial sirtuins (Sirt3, Sirt4, Sirt5) in VAT adipocytes and BAT (n = 3–5 per group). C: Western blot analysis of SIRT3, acetylated lysine (Ac-K), and cytochrome c oxidase (COX) IV. Mitochondrial proteins isolated from VAT adipocytes and BAT were separated on Any-kD gels (Bio-Rad). Densitometric analysis of acetylated mitochondrial protein bands (between 20 and 50 kDa) is shown. *Value significantly different from control value (Student’s t-test, P < 0.05). Values are means ± SE.