Table 1.
Sequence of primers for real-time PCR
| Primer Sequences (5′-3′) | |||
|---|---|---|---|
| Gene | Accession Number | Forward | Reverse |
| Sirt3 | NM_001177804 | ATCCCGGACTTCAGATCCCC | CAACATGAAAAAGGGCTTGGG |
| Sirt4 | NM_001167691 | GATTGACTTTCAGGCCGACAA | GCGGCACAAATAACCCCGA |
| Sirt5 | NM_178848 | CTCCGGGCCGATTCATTTCC | GCGTTCGCAAAACACTTCCG |
| Pgc1α | NM_008904 | TATGGAGTGACATAGAGTGTGCT | CCACTTCAATCCACCCAGAAAG |
| Adipoq | NM_009605 | TGTTCCTCTTAATCCTGCCCA | CCAACCTGCACAAGTTCCCTT |
| Lep | NM_008493 | GAGACCCCTGTGTCGGTTC | CTGCGTGTGTGAAATGTCATTG |
| Tnfα | NM_013693 | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG |
| Il6 | NM_031168 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
| Mcp1 | NM_011333 | TTAAAAACCTGGATCGGAACCAA | GCATTAGCTTCAGATTTACGGGT |
| Glut4 | NM_009204 | CTGTCGCTGGTTTCTCCAA | CTGCTCTAAAAGGGAAGGTGTC |
| Atgl | NM_001163689 | GGATGGCGGCATTTCAGACA | CAAAGGGTTGGGTTGGTTCAG |
| Hsl | NM_001039507 | CCAGCCTGAGGGCTTACTG | CTCCATTGACTGTGACATCTCG |
| Lcad | NM_007381 | TCTTTTCCTCGGAGCATGACA | GACCTCTCTACTCACTTCTCCAG |
| MnSOD | NM_013671 | CAGACCTGCCTTACGACTATGG | CTCGGTGGCGTTGAGATTGTT |
| Catalase | NM_009804 | AGCGACCAGATGAAGCAGTG | TCCGCTCTCTGTCAAAGTGTG |
| Foxo3a | NM_019740 | CTGGGGGAACCTGTCCTATG | TCATTCTGAACGCGCATGAAG |
| Idh2 | NM_173011 | GGAGAAGCCGGTAGTGGAGAT | GGTCTGGTCACGGTTTGGAA |
| Gpx1 | NM_008160 | AGTCCACCGTGTATGCCTTCT | GAGACGCGACATTCTCAATGA |
| Ucp1 | NM_009463 | AGGCTTCCAGTACCATTAGGT | CTGAGTGAGGCAAAGCTGATTT |
| Pparα | NM_011144 | AGAGCCCCATCTGTCCTCTC | ACTGGTAGTCTGCAAAACCAAA |
| Dio2 | NM_010050 | AATTATGCCTCGGAGAAGACCG | GGCAGTTGCCTAGTGAAAGGT |
| Cidea | NM_007702 | TGACATTCATGGGATTGCAGAC | GGCCAGTTGTGATGACTAAGAC |
| mtCO1 | YP_003024028 | GGATTTGTTCACTGATTCCCATTA | GCATCTGGGTAGTCTGAGTAGCG |
| Gapdh | NM_001289726 | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
| 36b4 | NM_007475 | GCAGACAACGTGGGCTCCAAGCAGAT | GGTCCTCCTTGGTGAACACGAAGCCC |