Fig. 2.

A: volcano plot displaying fold differences in individual proteins between mice consuming the low-fat diet (LFD) and mice consuming the high-fat diet (HFD) for 12 wk. Proteins were identified by high-resolution mass spectrometry of mitochondrial-enriched samples prepared from gastrocnemius muscle. Gene symbols are displayed for select proteins: Apoa, apolipoprotein A; Ascl2, achaete-scute family basic helix-loop-helix transcription factor 2; Ech1, enoyl-CoA hydratase 1; Etfdh, electron transfer flavoprotein dehydrogenase; Fabp3, fatty acid-binding protein 3; Hadhb, hydroxyacyl-CoA dehydrogenase trifunctional enzyme subunit β; Hsd17b4, hydroxysteroid 17β dehydrogenase 4; Prkag2, protein kinase AMP-activated noncatalytic subunit γ₂; Serpina1e, serpin family A member 1e; Skor1, Sk1 family transcriptional corepressor 1; Slc25a, solute carrier family 25a; Srp19, signal recognition particle 19. Horizontal dashed line represents statistical significance of P = 0.05 with n = 8 per diet group. B: relative quantification of mRNA by real-time polymerase chain reaction for select genes expressed relative to cyclophilin [for pyruvate dehydrogenase kinase 4 (Pdk4)] and heat shock protein 90 [for solute carrier family 25a member 20 (Slc25a20)] and compared by unpaired t-test. Individual data points are displayed along with mean (SD) (n = 8 per diet group).