Fig. 5.

Accumulation of oxidative or deamidation modification to amino acid residues of proteins identified by high-resolution mass spectrometry of mitochondrial-enriched samples collected from the gastrocnemius muscle of mice following 12 wk of low-fat diet (LFD) or high-fat diet (HFD) consumption. Oxidative damage and deamidation were compared between all proteins that were identified (A and B) and also separated to include only mitochondrial proteins (C and D). Bars represent mean (SD) (n = 8 per diet group). Differences between diet groups were compared by unpaired t-test. AU, arbitrary units.