Fig. 1.

Fibroblasti growth factor (Fgf) receptors (Fgfr), α-Klotho, and Fgf23 are expressed in skeletal muscle. A–C: are representative real-time RT-PCR amplification plots showing values of fluorescence at each cycle number of Fgfr1–4, α-Klotho, and Fgf23 (each reaction in triplicate) in isolated extensor digitorum longus (EDL; A) and soleus (B) muscles from 4- to 5-mo-old CD-1 male mice and in differentiated C₂C₁₂ myotubes (C). D: summary data showing average $2^{{}^{–\Delta C_T}}$ values (×10⁶) of Fgfr1–4, α-Klotho, and Fgf23 in EDL and soleus muscles from 4- to 5-mo-old CD-1 male mice (n = 3) and in C₂C₁₂ myoblasts and myotubes (n = 1) expressed relative to β-actin on log₁₀ scale.