Table 1.
Primers used for gene expression assay
| Gene Symbol | Gene Description | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|---|
| GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | TGGAGAAACCTGCCAAGTATGA | GAAGAGTGGGAGTTGCTGTTGA |
| iNOS | Inducible nitric oxide synthase | TGGTGGTGACAAGCACATTT | AAGGCCAAACACAGCATACC |
| Agr1 | Arginase 1 | AAAGCTGGTCTGCTGGAAAA | AGACCGTGGGTTCTTCAC |
| TNF-α | Tumor necrosis factor-α | GAACTGGCAGAAGAGGCACT | GGTCTGGGCCATAGAACTGA |
| IL-6 | Interleukin 6 | CCGGAGAGGAGACTTCACAG | TCCAGTTTGGTAGCATCCATC |
| IL-1β | Interleukin 1β | GGGCCTCAAAGGAAAGAATC | CTCTGCTTGTGAGGTGCTGA |
| CCL2 | C-C motif chemokine ligand 1 | AGCACCAGCCAACTCTCACT | CGTTAACTGCATCTGGCTGA |
| CXCL1 | Chemokine (C-X-C motif) ligand 1 | CTTGACCCTGAAGCTCCCTT | AGGTGCCATCAGAGCAGTCT |
| IL-10 | Interleukin 10 | CCAAGCCTTATCGGAAATGA | TTTTCACAGGGGAGAAATCG |
| IL-4 | Interleukin 4 | CCTCACAGCAACGAAGAACA | TTCAAGCATGGAGTTTTCCC |
| PAT2 | Proton/amino acid transporter 2 | GTGCCAAGAAGCTGCAGAG | TGTTGCCTTTGACCAGATGA |
| CD137 | Cluster of differentiation 37 | CGTGCAGAACTCCTGTGATAAC | GTCCACCTATGCTGGAGAAGG |
| UCP1 | Uncoupling protein 1 | CTTTGCCTCACTCAGGATTGG | ACTGCCACACCTCCAGTCATT |
| PRDM16 | PR domain 16 | CAGCACGGTGAAGCCATTC | GCGTGCATCCGCTTGTG |