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. 2018 Mar 6;315(4):E676–E693. doi: 10.1152/ajpendo.00224.2017

Fig. 9.

Fig. 9.

Kupffer cells (KCs), but not hepatocytes, express zinc finger protein 36 (Zfp36). A: vehicle and LPS-stimulated primary hepatocytes and bone marrow-derived macrophages (BMDM) isolated from loxP-flanked Zfp36 C57BL/6 [Zfp36fl/fl; Control (Ctrl)] and Zfp36fl/fl;LysMCre [myeloid knockout (KO)] mice were tested for Zfp36 protein expression by Western blotting using Cyclophilin B (CycloB) as loading control (compare with Fig. 1A). Positive control lane was 5 μg of cell lysate from 293T cells engineered to overexpress human ZFP36 cDNA. B: primary KCs express Zfp36 in response to LPS stimulation. Vehicle and LPS-stimulated primary KCs were tested for Zfp36 expression by Western blotting, as well as for Mac2 and Cyclophilin B expression (loading control). Positive control lane was 5 μg of cell lysate from 293T cells engineered to overexpress human ZFP36 cDNA. Data are representative of experiments performed 3 times.