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. 2018 Mar 6;315(4):E676–E693. doi: 10.1152/ajpendo.00224.2017

Fig. 10.

Fig. 10.

Loss of Kupffer cell-expressed zinc finger protein 36 (Zfp36) in obese mice is associated with enhanced hepatic IKKβ and p65 but not X-box-binding protein 1 (XBP1) nuclear activation. Whole liver lysates and liver nuclear extracts were isolated from lean and obese loxP-flanked Zfp36 C57BL/6 (Zfp36fl/fl; Control) and Zfp36fl/fl;LysMCre [myeloid knockout (KO)] mice and tested for protein expression. A: whole liver lysates from obese Control and KO mice demonstrate similar p65 and Xbp1 protein expression (with actin-loading control). B: nuclear lysates from obese KO mice have enhanced levels of IKKβ compared with obese Control mice or lean mice of both genotypes (H3 loading control). C: nuclear lysates from obese KO mice have enhanced levels of p65 compared with obese Control mice or lean mice of both genotypes (H3 loading control). D: hepatic nuclear Xbp1 expression is similar in obese Control and KO mice and higher than that observed in lean mice of both genotypes (H3 loading control). Data are representative of experiments performed 3 times.