Fig. 4.

Attenuated systemic vascular responses and attenuated vasoconstriction in renal tissue perfusion to angiotensin II (ANG II) infusion in female SMKO mice. A: average change in blood pressure after acute administration of sequential ANG II doses (0.10, 0.30, and 1.00 μg/kg) to anesthetized control (n = 11) and SMKO (n = 8) mice. Values are means ± SE. Data were analyzed by 2-way repeated-measures ANOVA with Sidak’s post hoc test for multiple comparisons. ANOVA table for genotype: F(1,17) = 23.15, P < 0.001; Sidak’s post hoc test: P = not significant for 0.10 μg/kg, ***P < 0.001 for 0.30 and 1.00 μg/kg. B: average change in heart rate after acute administration of sequential ANG II doses in anesthetized control (n = 11) and SMKO (n = 8) mice. Values are means ± SE. Data were analyzed by 2-way repeated-measures ANOVA with Sidak’s post hoc test for multiple comparisons. ANOVA table for genotype: F(1,17) = 16.98, P = 0.0007; Sidak’s post hoc test: P = not significant for 0.10 μg/kg, ***P = 0.0008 for 0.30 μg/kg, *P < 0.01 for 1.00 μg/kg. C: baseline kidney tissue perfusion in control (n = 11) and SMKO (n = 8) mice (P = 0.053). Values (means ± SE) are shown as tissue perfusion units (TPUs). Data were analyzed by unpaired t-test. D: reduction in mean kidney tissue perfusion was compared with respective baseline values in response to increasing concentrations (0.03, 0.1, and 0.30 μg/kg) of ANG II. Baseline changes were compared between SMKO (n = 8) and control (n = 11) mice. Values are means ± SE. Data were analyzed by 2-way repeated-measures ANOVA with Sidak’s post hoc test for multiple comparisons. ANOVA table for genotype: F(1,17) = 48.28, P < 0.0001; Sidak’s post hoc test: P = not significant for 0.03 μg/kg, ***P < 0.0001 for 0.10 and 0.30 μg/kg.