Fig. 2.

Nestin-Cre did not cause ferroportin (FPN) deletion in duodenal epithelia of floxed FPN (FPN^f/f)/Nestin-Cre mice. A: PCR genotyping showed that the FPN gene was deleted in some duodenal cells of FPN^f/+/Nestin-Cre and FPN^f/f/Nestin-Cre mice. B: immunofluorescence showed that expression of FPN protein in duodenal epithelia of FPN^f/f/Nestin-Cre mice was intact and comparable to WT counterparts. C: Perl’s staining showed comparable iron accumulation in duodenal epithelia of WT and FPN^f/f/Nestin-Cre mice. Scale bars, 50 μm.