Fig. 10.

Ferroportin (FPN) knockout (KO) by Nestin-Cre resulted in decreased serum and liver iron and increased renal iron in ischemic acute kidney injury (AKI) mice. Serum iron (A), transferrin saturation (TSAT; B), and liver iron (C) were decreased, and kidney iron was increased (C) in floxed FPN (FPN^f/f)/Nestin-Cre vs. WT ischemic AKI mice. D: spleen iron was comparable in FPN^f/f/Nestin-Cre and WT ischemic AKI mice. E and F: FPN and divalent metal transporter 1(iron-responsive element) [DMT1(IRE)] mRNA were comparable in duodenums of FPN^f/f/Nestin-Cre and WT ischemic AKI mice, respectively. Data are presented as means ± SE (3 mo; n = 6, male) and were analyzed using Student’s t-test.