Table 1.

List of antibodies used

Antibody	Species	Dilution for WB	Dilution for IF	Source	Ref. No.	Used in Figure No.
pT53-NCC*	Rabbit	1:2,000	1:10,0000	Ellison Laboratory, R40	21	1, 4
NCC*	Rabbit	1:6,000	1:7,500	Ellison Laboratory	2	1, 4
pT96, pT101-NKCC2	Rabbit	1:1,000	1:1,000	Bachmann Laboratory	25	1, 4, 7
NKCC2	Guinea Pig	1:3,000	1:8,000	Bachmann Laboratory	38	1, 3, 4, 7
pS91-NKCC2	Sheep	3 µg/ml	1 µg/ml	MRC PPU, Dundee Univ. S838B	36	4, 5
NKCC2	Sheep	3 µg/ml		MRC PPU, Dundee Univ. S451C	34	5
WNK4*	Rabbit		1:1,000	Ellison Laboratory	46	3
AQP2	Goat		1:1,000	Santa Cruz sc-9882		3
Parvalbumin (PV)*	Guinea Pig		1:2,000	Swant GP72	7	4
Calbindin D-28K (C28)	Mouse		1:2,000	Swant 300	4	4

^*Validated in knockout mice.