A: Western blot analysis of collecting duct (CD) epithelial Na+ channel (αENaC) protein expression following 24 h of systemic 5% dextrose in water (sD5W) + renal interstitial (RI) D5W (open bar, group 1), systemic angiotensin II (sANG II) + RI scrambled (SCR) siRNA (solid bar, group 3), and sANG II + RI ghrelin receptor (GR) siRNA (shaded bar, group 4) infusions. B: Western blot analysis of total kidney αENaC protein expression in response to the same treatments in A. All blots were normalized to β-tubulin. Data represent means ± SE. *P < 0.05 from sD5W + RI D5W and sANG II + RI GR siRNA treatments.