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. 2018 Aug 6;99(9):1187–1198. doi: 10.1099/jgv.0.001128

Fig. 7.

Fig. 7.

PR8_HA(D-MVEKT) but not PR8_HA(MVEKT) virus infection results in severe bronchiolar epithelial damage in Tmprss2−/− mice. Female 8–12-week-old WT and Tmprss2−/− KO mice were infected intranasally with 2×105 f.f.u. PR8 (WT: n=3; KO: n=3), PR8_HA(HK) (WT: n=3; KO: n=3), PR8_HA(MVEKT) (WT: n=3; KO: n=3) and PR8_HA(D-MVEKT) (WT: n=3; KO: n=3). Lungs were prepared at day 4 p.i., sectioned and stained with H&E. PR8 and all recombinant viruses caused severe damage of bronchial epithelium (inserts) in the lungs from WT mice. In contrast, in Tmprss2−/− mice regions of severely damaged epithelial layers (inserts) were mainly observed in PR8_HA(HK) and PR8_HA(D-MVEKT) infected animals, whereas PR8 and PR8_HA(MVEKT) only produced mild or no damage of bronchial epithelium (inserts). Note also the similar degree of inflammatory cell infiltration in all infected mice. Bars, 200 µm.