Fig. 2.

Effect of L-type blocker 5 µM nifedipine (Nif) on afterhyperpolarizations (AHPs) and corresponding Ca²⁺ transients in oxytocin (OT) (n = 5) (A–D) and vasopressin (VP) (n = 5) (E–H) neurons. A: example AHP after a 20-Hz, 20-spike train from an OT neuron treated with Nif and the corresponding somatic Ca²⁺ signal. B: example AHP after a 20-Hz, 5-spike train from an OT neuron treated with Nif and its corresponding somatic Ca²⁺ signal. C: summary data for OT neuron AHP measurements at the 20-spike peak amplitude (medium and slow AHP, mAHP + sAHP), at 1 s after the train (sAHP), and 5-spike AHPs at the peak amplitude (mAHP). Red points are individual cells, and black points are group averages (paired t-test; P > 0.05 for all 3 measurements). D: summary data for OT neuron Ca²⁺ transients. Nif significantly reduced peak %ΔF/F in 20-spike AHPs (**P < 0.01) and 5-spike AHPs (*P < 0.05). E: example AHP after a 20-Hz, 20-spike train from a VP neuron treated with Nif and its corresponding somatic Ca²⁺ signal. F: example AHP after a 20-Hz, 5-spike train from a VP neuron treated with Nif and its corresponding somatic Ca²⁺ signal. G: summary data for VP neuron AHP measurements at the 20-spike peak amplitude (mAHP + sAHP), at 1 s after the train (sAHP), and 5-spike AHPs at the peak amplitude (mAHP). Green points are individual cells, and black points are group averages (paired t-test; P > 0.05 for all 3 measurements). H: Nif significantly reduced peak %ΔF/F from 20-spike trains (**P < 0.01) and 5-spike trains (*P < 0.05). All data were evaluated with a 2-way repeated-measures ANOVA.