Fig. 4.
ACh/At-mediated changes in spontaneous excitatory postsynaptic current (sEPSC) frequencies in external tufted (ET) and periglomerular (PG) cells. A: representative 10-s traces from PG and ET cells held at Vm = −70mV and given a 1 s ACh/At puff in slices from wild-type (WT) animals. Expanded sections following ACh application are shown in blue boxes (scales given by blue scale bars). B: frequency vs. time plots for sEPSC events in both cell types and each knockout group. Gross frequencies were determined within 1 s bins throughout the recordings and averaged between cells. A 1 s ACh/At puff was applied at 30 s (blue bar). C: ACh effect on mean sEPSC frequencies across both cell types and knockout conditions. Baseline frequencies were averaged over 30 s before ACh application, and ACh frequencies were averaged over 5 s from the beginning of application. D: comparison of net changes in mean sEPSC frequencies between knockout types (*P < 0.05, ns = no statistical significance). ACh/At, 1 mM acetylcholine in the presence of 2 µM atropine.