Fig. 3.

Representative immunoblots for endothelial NOS (eNOS) and β-actin in pulmonary artery endothelial cells (PAEC) from control and PPHN lambs and summarized data (A, n = 5) showing decrease in eNOS protein in PPHN. IOD, integrated optical density. The mRNA levels of eNOS were decreased in PPHN (B, n = 5). Levels of cGMP, an indicator of NOS function, were decreased in PPHN at basal level. Stimulation with NOS agonist ATP increased cGMP levels in control, but not PPHN cells (C, n = 4). *P < 0.05 from control by unpaired t-test.