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. 2018 Aug 17;50(10):862–875. doi: 10.1152/physiolgenomics.00076.2018

Fig. 4.

Fig. 4.

Pgf−/− mouse performance on the serial dishabituation test (SDT). In the first trial of the SDT, Pgf+/+ and Pgf−/− mice spent similar amounts of time moving (A), although Pgf−/− mice reared fewer times (B). The time spent in the center of the arena as a percentage of total time was also similar between Pgf+/+ and Pgf−/− mice (C). Pgf+/+ and Pgf−/− mice spent similar amounts of time exploring the objects in each trial (D), although Pgf−/− mice tended to make fewer visits to the objects (E). Pgf−/− mice had a lower total number of visits over the entire SDT (F). Pgf+/+ and Pgf−/− mice exhibited a significantly greater increase in time investigating the changed object compared with the unchanged objects during the New Location trial (G). Only Pgf−/− mice spent significantly greater time investigating the changed object in the Substitution trial (H). Neither the Pgf+/+or Pgf−/− had significantly greater increases in exploration of the changed objects during the Spatial Switch (I) or Addition (J) trials. Each mouse underwent 11 consecutive trials in the SDT. The first trial allowed accommodation to the empty arena and was used to analyze activity and anxiety-like behavior. The second to fourth trials familiarized the mice with an array of objects. The geometry of the array was distorted (New Location, Addition) or objects were exchanged without altering the geometry (Spatial Switch, Substitution) in later trials. Changes in the time spent investigating changed and unchanged objects relative to the baseline trial experienced directly previously are presented. Data were analyzed with unpaired, two-tailed t-tests, Mann-Whitney tests and two-way repeated-measures ANOVAs. Graphs show means ± SD. Circles represent Pgf+/+ mice, while squares represent Pgf−/− mice. Black bars show the change in time spent investigating the changed object(s), while light gray bars show the change in time investigating the unchanged objects; n = 23 Pgf+/+ and 20 Pgf−/− male and female mice. *P < 0.05, **P < 0.01, ****P < 0.0001.