Table 4.
Mature miRNA regulators upstream of changed genes in NHE1 transgenic hearts
| Upstream Regulator | Expr Fold Change | Expr P Value | Predicted Activation State | Bias-corrected z-score | Activation z-score | Flags | P Value of Overlap | Target Molecules in Data Set |
|---|---|---|---|---|---|---|---|---|
| miR-16-5p | −1.825 | 0.00E +00 | inhibited | −5.842 | bias | 1E-08 | ACP2, ACTR1A, BCL2, CAPRIN1, CCND1, CFL2, DMTF1, DNAJB4, GOLPH3L, GRN, GTF2H1, H3F3A/H3F3B, HARS, IGF1, IGF2R, KPNA3, LAMC1, LAMTOR3, MAP2K1, MCL1, MRPL20, NPR3, PAFAH1B2, PHLDB2, PISD, PURA, RARS, RTN4, SERPINE2, SKAP2, SLC12A2, SPTLC1, SQSTM1, TMEM109, TPI1, TPPP3, UGDH, UGP2, VTI1B | |
| miR-124-3p | * | inhibited | −5.168 | bias | 2E-06 | ACAA2, ARFIP1, ARPC1B, ATL3, ATP6V0E1, BLOC1S6, CTDSP2, CTGF, CTNND1, F11R, FCHO2, GSN, HADH, HES1, HTATIP2, ITGB1, KLF15, LAMC1, MDFIC, MMP2, MTMR6, MTPN, NECAP2, PGRMC2, POLR3G, PTTG1IP, R BMS1, ROCK1, RYK, SERPINB6, SLC50A1, SSFA2, STAT3, SWAP70, TLN1, TMBIM1, TMEM109, ZNF367 | ||
| miR-29b-3p | −1.641 | 0.00E +00 | inhibited | −3.429 | bias | 5E-06 | ARPC3, COL15A1, COL3A1, COL4A2, FBN1, GMFB, KLF4, LAMC1, MCL1, PMP22, PPIC, PPM1D, PURA, SPARC, TGFB3, TUBB2A | |
| miR-30c-5p | −2.923 | 0.00E +00 | inhibited | −3.845 | bias | 1E-05 | ATP2A2, BECN1, CTGF, ELMOD2, GALNT1, GNAI2, MAT2A, NPR3, NT5E, PAFAH1B2, PRPF40A, PTGFRN, RBMS1, SEC23A, SEC62, SLC38A2, STRN, STX7, TMED2, TMED7, TMEM59, WDR92 | |
| miR-17-5p | −1.300 | 8.44E-07 | inhibited | −1.456 | −3.275 | bias | 5E-05 | APP, BCL2, BIRC5, BMPR2, CCND1, CRIM1, PURA, RGS5, RHOA, RHOC, S1PR1, STAT3, TGFBR2, VIM |
| miR-192-5p | −1.426 | 0.00E +00 | 1.453 | 0.603 | bias | 9E-05 | BIRC5, DHFR, IGF1, TYMS, ZEB1 | |
| miR-199a-5p | 2.547 | 0.00E +00 | inhibited | −2.376 | bias | 0.0001 | ACTA1, ALOX5AP, BGN, BIRC5, C3, DCN, FN1, HIF1A, ITGA1, KLF4, PIGR, SAMD9L, TGFBR1, TGFBR2, ZBTB16 | |
| miR-155-5p | −1.686 | 8.36E-14 | inhibited | −3.418 | bias | 0.0007 | ARFIP1, ATP6V1C1, CCND1, CYR61, DSG2, FAR1, IL13RA1, KRAS, MATR3, MOSPD2, NARS, NT5E, PDLIM5, PICALM, POLE3, RHOA, SDCBP, SERPINE1, TBCA, TCF7L2, TNFRSF10A, TXNRD1, WDFY1 | |
| miR-542-3p | 1.723 | 3.73E-08 | −1.955 | bias | 0.0008 | ILK, RPL11, RPL22, RPS23 | ||
| miR-199a-3p | inhibited | −2.433 | bias | 0.0009 | CALU, CD44, FN1, ITGA6, PON2, VCAN | |||
| miR-21-5p | 3.851 | 0.00E +00 | inhibited | −2.694 | bias | 0.001 | ACTA2, BMPR2, BTG2, C8orf44-SGK3/SGK3, CFL2, MMP2, PECAM1, SERPINB5, STAT3, TCF21, TGFB2, TGFBR2 | |
| miR-19b-3p | −1.185 | 4.89E-03 | −0.544 | −1.506 | bias | 0.0016 | BIRC5, BMPR2, CCND1, CTGF, THBS1 | |
| miR-291a-3p | inhibited | −3.365 | bias | 0.0016 | ADAM9, APP, C3, CCND1, CD44, CFL2, FAM13B, FYCO1, INSIG2, KLHL12, NUP58, PCGF5, STX11, UBXN1, ZHX1 | |||
| miR-1-3p | 6.451 | 0.00E +00 | inhibited | −4.083 | bias | 0.0019 | ANXA2, AP3B1, AXL, BCL2, CAP1, CNN3, CORO1C, FBLN2, FSTL1, GNPDA2, H3F3A/H3F3B, IGF1, IP6K2, KCNJ2, LRP1, PICALM, PPIB, SDC4, SERPINB5, SLC44A1, SNX6, TAGLN2, THBS1, Tmsb4x (includes others), WDFY1, YWHAQ | |
| miR-293-5p | −1.664 | bias | 0.0031 | AKAP6, GOLPH3, KLF15, MGST1, NR4A1, SSR3 | ||||
| miR-34a-5p | 2.578 | 0.00E +00 | −1.895 | bias | 0.0038 | BCL2, BIRC5, CCND1, CDK1, DHFR, ICAM1, IKBIP, KLF4, MAP2K1, STIM1 | ||
| miR-451a | −1.593 | 0.00E +00 | −1.998 | bias | 0.0041 | BCL2, CCND1, MIF, MMP2 | ||
| miR-1195 | * | 0.0053 | IGF1, STAT3 | |||||
| miR-129-5p | −1.969 | bias | 0.0061 | BMPR2, CAMTA1, GALNT1, PDS5A | ||||
| let-7a-5p | 1.324 | 0.00E +00 | inhibited | −3.219 | bias | 0.0062 | ACTA2, BIRC5, CCND1, CHMP2A, COL3A1, CSDE1, ITGA1, ITGA4, KRAS, MTPN, PGRMC1, SLC25A32, SMOX, SPCS3, STAT3, TGFBR1, THBS1, TLR4, TYMS, VIM | |
| miR-320b | 0.0108 | IGF1, MCL1, VIM | ||||||
| miR-138-5p | * | 0.0108 | RHOC, ROCK2, VCAN | |||||
| miR-153-3p | −2.773 | 4.73E-07 | 0.0151 | BCL2, MCL1 | ||||
| miR-193a-3p | * | 0.0163 | CCND1, MCL1, PTK2 | |||||
| miR-30a-3p | −1.132 | 0.00E +00 | inhibited | −2.236 | bias | 0.0226 | CYR61, RAB8B, RSU1, THBS1, TUBA1A | |
| miR-149-5p | −1.622 | 0.00E +00 | 0.0288 | RAP1A, RAP1B | ||||
| miR-125b-5p | 1.230 | 0.00E +00 | inhibited | −2.224 | bias | 0.0297 | ACSS1, ADAMTS1, GSS, H3F3A/H3F3B, ID2, IGFBP3, LIPA, MAN1A1, MAP2K1, PIGR, SGPL1, VPS4B | |
| miR-133a-3p | −2.692 | 0.00E +00 | −1.875 | bias | 0.0299 | BTBD3, Cdc42, CORO1C, CTGF, KLF15, MCL1, MSN, RHOA | ||
| miR-200b-3p | * | −0.39 | −1.444 | bias | 0.0317 | ERBIN, ERRFI1, VIM, WDR37, ZEB1 | ||
| miR-135a-5p | −1.168 | 3.25E-01 | 0.0408 | ALOX5AP, APC, JAK2 |
*
Low abundance.