Fig. 3.

Lung miR-26a, EphA2, KDR, and ROCK1 after intratracheal (IT) bleomycin. Mice were treated with 100 µl of PBS or 0.1 U of bleomycin in 100 µl of PBS. Lungs were harvested 4 and 7 days after treatment. A: lung miR-26a at 4 and 7 days. Lung EphA2 (B), KDR (C), and ROCK1 (D) mRNA levels at 7 days. E: relative densitometry for Lung EphA2 protein at 7 days with a representative Western blot image. Data displayed as means ± SE; n = 5–11, *P < 0.05 compared with PBS by unpaired t-test. U6, small nuclear RNA U6; 18S, ribosomal protein 18S. F: Western blot for lung tissue with EphA2 band at 132 kDa and nonspecific lower MW bands (not included in densitometry). G: Western blot for cell protein with EphA2 band at 132 kDa and nonspecific lower MW bands. Black arrowhead denotes  EphA2.