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. 2018 Jul 19;315(4):L584–L594. doi: 10.1152/ajplung.00073.2017

Fig. 4.

Fig. 4.

miR-26a regulates EphA2 expression in human lung microvascular endothelial cells (HMVEC-L). Cells were transfected with miR-26a mimic, inhibitor, or respective negative control (NC) and harvested at 72 h. miR-26a levels after transfection with miR-26a inhibitor (A) and mimic (B). EphA2 mRNA levels after transfection with miR-26a inhibitor (C) and mimic (D). Relative densitometry of EphA2 protein levels after transfection with miR-26a inhibitor (E) and mimic (F) with representative Western blots. KDR mRNA levels after transfection with miR-26a inhibitor (G) and mimic (H). ROCK1 mRNA levels after transfection with miR-26a inhibitor (I) and mimic (J). Data are displayed as means ± SE; n = 5–8, *P < 0.05 compared with NC determined by unpaired t-test. U6, small nuclear RNA U6; 18S, ribosomal protein 18S.