Table 3.
Fold increase RNA expression in sorted EPI, END, MES, and MIC cell populations compared with PMX, dissociated cells by qRT-PCR
| EPI | END | MES | MIC | |
|---|---|---|---|---|
| EpCAM | 37.3 | 0.3 | 0.6 | 0.1 |
| SFTPC | 77.6 | 0.02 | 0.1 | 0.4 |
| SCGB1A1 | 47.5 | 0.2 | 0.2 | 0.06 |
| RAGE/AGER | 11.9 | 0.98 | 1.7 | 0.32 |
| CD45 | <0.01 | <0.01 | 0.05 | 2.72 |
| CD14 | 0.08 | 0.36 | 0.12 | 2.29 |
| CD31/PECAM | 0.03 | 5.14 | 0.05 | 0.36 |
| CD144/VE-Cadherin | 0.02 | 9.6 | 0.02 | 0.05 |
| VWF | 0.02 | 15.8 | 0.03 | 0.04 |
| ACTA2 | 4.76 | 0.19 | 7.81 | 0.06 |
| PDGFRβ | 4.5 | 0.22 | 10.95 | 0.05 |
| ELN | 7.93 | 1.57 | 11.66 | 0.03 |
ACTA2, smooth muscle actin; CD, cluster of differentiation; ELN, elastin; END, endothelial cell population; MIC, mixed immune cell population; EpCAM, epithelial cell adhesion molecule; EPI, epithelial cell population; MES, nonendothelial mesenchymal cell population; PDGFR, platelet-derived growth factor receptor; PECAM, platelet endothelial cell adhesion molecule; PMX, presort mixed cells; qRT-PCR, quantitative RT-PCR; RAGE/AGER, receptor for advanced glycation endproducts/advanced glycosylation end-product specific receptor; SCGB1A1, secretoglobin family 1A member 1; SFTPC, surfactant protein C; VE-cadherin, vascular endothelial cadherin; VWF, von Willebrand factor.