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. 2018 May 23;315(4):R627–R637. doi: 10.1152/ajpregu.00364.2017

Fig. 4.

Fig. 4.

Short-term thermoneutral housing reduces gene expression markers of thermogenesis in brown adipose tissue (BAT). Ucp1 (A), Prdm16 (B), Cox8b (C), and Ppargc1a (D) following 1-day control (CON), 1-day high-fat diet (HFD), 5-day CON, and 5-day HFD treatment at standard vivarium temperature (22°C) and murine thermoneutral temperature (30°C). Immunoblots (E) and quantification of PRDM16 (F) and uncoupling protein 1 (UCP-1) (G) abundance in BAT. Data reported as means ± SE. Data were analyzed by a two-way ANOVA (diet × temperature) with a Sidak multiple comparison test. P values for main effects (P ≤ 0.01) are listed in each graph. #Significant findings with multiple comparison test for temperature (#P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001); and *significant findings with multiple comparison test for diet (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). NS, not significant.