Standard vivarium housing temperature (Temp) (22°C) but not thermoneutral housing increases epididymal white adipose tissue (eWAT) proinflammatory gene expression in response to an high-fat diet (HFD). eWAT Tnf (A), Il1b (B), Il6 (C), and Ccl2 (D) gene expression following 1-day CON, 1-day HFD, 5-day control (CON), and 5-day HFD at standard vivarium temperature (22°C) and murine thermoneutral temperature (30°C). Data reported as means ± SE. #Significant findings with multiple comparison test for temperature (#P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001); and *significant findings with multiple comparison test for diet (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).