Table 3.
Effect of glutamate receptor blockade in DMH on POA cooling-evoked responses
| Precooling |
POA Cooling |
Postinjection |
||||
|---|---|---|---|---|---|---|
| Vehicle | AP5/CNQX | Vehicle | AP5/CNQX | Vehicle | AP5/CNQX | |
| TPOA, °C | 35.0 ± 0 | 35.3 ± 0.2 | 30.0 ± 1.0* | 31.2 ± 0.9* | 31.0 ± 1.0* | 31.6 ± 0.7* |
| BAT SNA, %baseline | 100 ± 1 | 100 ± 1 | 610 ± 1.0* | 738 ± 96* | 602 ± 1* | 158 ± 26‡ |
| TBAT, °C | 33.6 ± 0.5 | 34.3 ± 0.3 | 34.9 ± 0.5* | 35.7 ± 0.2* | 35.1 ± 0.5* | 34.7 ± 0.2‡ |
| Exp. CO2, % | 4.8 ± 0.3 | 4.8 ± 0.1 | 5.2 ± 0.3* | 5.2 ± 0.1* | 5.3 ± 0.3* | 4.8 ± 0.1‡ |
| HR, beats/min | 395 ± 13 | 378 ± 19 | 442 ± 19* | 427 ± 24* | 443 ± 16* | 377 ± 20‡ |
| MAP, mmHg | 118 ± 3 | 108 ± 13 | 115 ± 3 | 108 ± 10 | 116 ± 4 | 101 ± 11 |
| Tcore, °C | 36.8 ± 0.5 | 38.0 ± 0.0 | 37 ± 0.5 | 38.0 ± 0.0 | 37.1 ± 0.5 | 38.0 ± 0.0‡ |
| Tskin, °C | 38.0 ± 0.5 | 38.0 ± 0.5 | 38.0 ± 0.5 | 38.0 ± 0.5 | 37.9 ± 0.5 | 37.9 ± 0.5 |
Values are means ± SE for preoptic area temperature (TPOA), brown adipose tissue (BAT) sympathetic nerve activity (SNA), BAT temperature (TBAT), expired carbon dioxide (Exp. CO2), heart rate (HR), mean arterial pressure (MAP), core temperature (Tcore), and skin temperature (Tskin) before preoptic area (POA) cooling, during cooling (5 min after beginning cooling), and 5 min after injection of either saline vehicle or (2R)-amino-5-phosphonovalericacid (AP5)/6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) in the dorsomedial hypothalamus (DMH).
*
P < 0.05, significantly different from the precooling values.
‡
Significantly different from vehicle-treated group.