Fig. 5.

Image analyses of the phosphorylated (p)AMP-activated protein kinase-α (AMPK-α)-to-AMPK-α ratio (A) and peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α expression (B) as determined by densitometry in the cytosol and in the nucleus of hearts of control mice under basal conditions. AICAR, 5-amino-4-imidazole carboxamide riboside. Data represent means ± SE of 4 mice/group and are expressed as relative intensity units. *P < 0.05 vs. baseline values of age-matched control mice; #P < 0.05 vs. age-matched mice treated with vehicle; ‡P < 0.05 vs. young mice as determined by ANOVA on ranks test.