Table 2.
MicroRNAs with significant differences between control and shunt RVs
| Target | Fold Change (Relative to Control) | P Value |
|---|---|---|
| miR-378a-3p | 0.38* | 0.024* |
| miR-208a-3p | 0.41* | 0.016* |
| miR-101-3p | 0.44* | 0.008* |
| miR-199b-5p | 0.60* | 0.042* |
| miR-28-5p | 1.43 | 0.024 |
| miR-25-3p | 1.44 | 0.033 |
| miR-152-3p | 1.56 | 0.012 |
| let-7e-5p | 1.61 | 0.042 |
| miR-23a-3p | 1.76 | 0.043 |
| miR-22-3p | 1.80 | 0.018 |
| miR-143-3p | 1.83 | 0.029 |
| miR-379-5p | 1.88 | 0.044 |
| miR-99b-5p | 1.91 | 0.018 |
| miR-106b-5p | 1.92 | 0.048 |
| miR-92a-3p | 2.12 | 0.009 |
| miR-27b-3p | 2.18 | 0.016 |
| miR-7-5p | 2.33 | 0.018 |
| miR-374a-5p | 2.47 | 0.006 |
| miR-191-5p | 2.58 | 0.003 |
| miR-221-3p | 2.58 | 0.036 |
| miR-208b-3p | 2.59 | 0.004 |
| miR-15a-5p | 2.69 | 0.035 |
| miR-128-3p | 2.78 | 0.001 |
| miR-99a-5p | 2.85 | 0.041 |
| miR-185-5p | 2.95 | 0.002 |
| miR-543 | 3.32 | 0.012 |
| miR-222-3p | 3.47 | 0.009 |
| miR-379-3p | 3.50 | 0.042 |
| let-7f-5p | 5.52 | 0.037 |
| miR-18a-5p | 7.33 | 0.027 |
| miR-338-3p | 7.46 | 0.045 |
| miR-212-5p | 9.23 | 0.033 |
| miR-29a-3p | 10.98 | 0.016 |
| miR-664a-3p | 15.72 | 0.016 |
| miR-382-5p | 19.19 | 0.008 |
| miR-1248 | 20.93 | 0.015 |
| miR-191-3p | 103.10 | 0.010 |
| miR-200b-3p | 133.40 | 0.007 |
| miR-34c-5p | 461.97 | 0.004 |
A total of 40 (of 93 tested) microRNA (miRNA) targets were significantly different (P < 0.05) between control and shunt right ventricular (RV) tissue. *Downregulated compared with control. ΔΔCT (where CT is threshold cycle) was calculated using let-7a-5p as a reference and normalized to compare relative miRNA expression between shunt and control tissue (n = 5); UniSp6 was used as a loading control per protocol.