Figure 10.

Changing ROS level contributing to different autophagic capacity. (a‐c) In vivo and vitro detection of H₂O₂ level in the 14th day under drought stress. (d‐e) MDC staining (d) and autophagic activity (e) in different treatments of transgenic tomatoes in the 14th day under drought condition. (f‐h) ATG8d (c), ATG18h (d) and ATG8 protein levels expression level in different treatments of transgenic tomatoes in the 14th day under drought condition. ATG8 and ATG8‐PE are the nonlipidated and lipidated forms of ATG8, respectively. The β‐actin was used as a loading control for the Western blotting analysis. Exogenous H₂O₂ at or DMTU was sprayed onto tomato plants every 3 d under drought condition. Means with the same letter did not significantly differ at P < 0.05 according to Duncan multiple range tests. Three independent experiments were performed with similar results.