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. 2018 May 29;16(12):2053–2062. doi: 10.1111/pbi.12938

Figure 1.

Figure 1

Comparison of the sensitivities of mutation detection by PCR/RNP, PCR/T7EI and direct Sanger sequencing. (a) The sg‐OsPDS‐1 target site is located in exon1 of OsPDS . The PAM sequence is highlighted in red. ‘D1’ indicates a 1 bp deletion at the target site. (b & c) Mixtures of WT and D1 PCR amplicons in different ratios were treated by PCR/RNP and PCR/T7EI. (d) DNA sequence of the PCR amplicons surrounding the sg‐OsPDS‐1 target site. The sgRNA sequence and 12 consecutive T's in the amplicons are highlighted in red and blue, respectively. (e) Mixtures of different proportions of WT and D1 PCR amplicons sequenced by the Sanger method.