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. 2018 May 29;16(12):2053–2062. doi: 10.1111/pbi.12938

Figure 5.

Figure 5

Genotyping base editing mutations by the PCR/RNP method. (a & b) The OsPDS‐1 and OsPDS‐4 target sites in exon1 and exon12 of OsPDS , respectively. The PAM sequences of FnCpf1 and SpCas9 are highlighted in blue and red, respectively. Candidate C to T and A to G conversions in the base editing windows are listed. (c & d) Analysis of base editing mutations in both target sites induced by FnCpf1 and the high‐fidelity SpCas9 variants.