Table 1.
Amino acid profiling analysis of control and CGS‐RNAi transgenic switchgrass plants
| Amino acids | Fold change (CGSRi‐1/Ctrl) | P‐value | Fold change (CGSRi‐3/Ctrl) | P‐value |
|---|---|---|---|---|
| Phenylalanine | 3.19 | 7.7E‐07 | 2.58 | 1.1E‐05 |
| Tryptophan | 3.51 | 8.6E‐05 | 3.10 | 0.00034 |
| Creatinine | 0.67 | 0.01790 | 0.81 | 0.16473 |
| Isoleucine | 1.62 | 0.00678 | 1.71 | 0.00291 |
| Methionine | 1.79 | 0.11575 | 1.94 | 0.06194 |
| Tyrosine | 2.66 | 9.2E‐06 | 2.27 | 8.0E‐05 |
| Valine | 2.56 | 6.9E‐05 | 2.20 | 0.00047 |
| Proline | 2.24 | 0.00016 | 2.09 | 0.00045 |
| Leucine | 1.20 | 0.52888 | 1.21 | 0.52120 |
| Hydroxyproline | 6.18 | 0.03141 | 7.64 | 0.01203 |
| Threonine | 8.53 | 0.00154 | 8.44 | 0.00167 |
| Alanine | 4.62 | 2.5E‐05 | 3.36 | 0.00065 |
| Creatine | 0.53 | 0.00594 | 0.54 | 0.00722 |
| Glutamine | 5.92 | 0.00082 | 5.28 | 0.00210 |
| Glutamate | 1.30 | 0.44510 | 2.17 | 0.00267 |
| Glycine | 8.18 | 0.00015 | 6.16 | 0.00159 |
| Serine | 6.99 | 5.2E‐07 | 5.00 | 1.5E‐05 |
| Asparagine | 51.38 | 3.9E‐08 | 54.48 | 3.5E‐08 |
| Citrulline | 3.39 | 0.35298 | 7.09 | 0.01396 |
| Aspartate | 3.34 | 0.01402 | 5.34 | 0.00025 |
| Histidine | 3.69 | 8.4E‐05 | 3.62 | 0.00010 |
| Arginine | 3.68 | 8.7E‐06 | 3.96 | 3.8E‐06 |
| Lysine | 8.90 | 0.01069 | 10.18 | 0.00441 |
| Ornithine | 1.38 | 0.86377 | 3.23 | 0.04223 |
| Putrescine | 1.75 | 0.03909 | 2.20 | 0.00300 |
| Spermidine | 1.12 | 0.62225 | 1.07 | 0.82758 |
Switchgrass stems at E4 stage were collected for profiling analysis of amino acids using LC‐MS/MS. The control plants (Ctrl) were produced with pANIC8B empty vector from the same batch of experiment. Three technical replicates and two biological replicates were conducted for amino acid profiling. The mean values were used for statistical analyses. Aspartate‐derived and aromatic amino acids are shown in bold font.