Table 1.
Expression of cytosolic DNA sensors mRNA after transfection of B16 cells with pEGFP with two different transfection methods; K2® Transfection System (K2) and electroporation (EP). Combination of EP with the cocktail was also tested.
| B16F1 | B16F10 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| N | DDX60 | DAI | p204 | IFN 1β | N | DDX60 | DAI | p204 | IFN 1β | |
| CONTROL | 4 | 1.33±0.44 | 1.13±0.24 | 1.11±0.21 | 2.22±1.05 | 4 | 1.00±0.04 | 1.00±0.01 | 1.03±0.16 | 1.04±0.20 |
| pEGFP+K2 | 5 | 0.5±0.12 | 0.89±0.24 | 0.18±0.04* | 0.75±0.23 | 6 | 0.50±0.08* | 1.60±0.59 | 0.61±0.09* | 2.20±1.18 |
| CONTROL | 4 | 1.02±0.12 | 1.00±0.04 | 1.02±0.11 | 1.01±0.07 | 4 | 1.01±0.01 | 1.03±0.14 | 1.09±0.26 | 1.37±0.59 |
| pEGFP+EP | 5 | 15.63±3.43* | 42.77±11.08* | 45.79±12.20* | 15.30±7.24* | 5 | 4.08±0.55* | 5.83±0.92* | 3.80±1.07* | 15.30±7.29* |
| pEGFP+EP+cocktail | 6 | 3.42±1.20 | 2.17±0.47 | 3.03±0.31 | 7.22±3.22 | 6 | 0.75±0.43 | 0.69±0.15 | 0.89±0.12 | 0.97±0.49 |
Control, untreated cells; pEGFP+K2, pEGFP combined with K2 transfection system; pEGFP+EP, pEGFP combined with electroporation (EP); DAI, DNA-dependent activator of interferon regulator factor (Takaoka et al. 2007); DDX60, DEAD (Asp-Glu-Ala-Asp) box polypeptide 60 (Miyashita et al. 2011); p204 (Unterholzner et al. 2010); IFN 1β, Type I interferon; Arithmetic Mean±SEM,
*
statistically significant difference compared to control groups (p<0.05), N=number of samples in two independent experiment.