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. 2018 Oct 26;21:1302–1308. doi: 10.1016/j.dib.2018.10.078
Subject area Redox Biology, Neuroscience, Proteomics
More specific subject area Redox biology, Neurobiology, Aging, Protein homeostasis
Type of data Spreadsheets
How data was acquired Liquid chromatography / mass spectroscopy. Thermo Scientific LTQ Orbitrap XL or Thermo Scientific™ Q Exactive Plus equipped with an ultra-high performance liquid chromatography unit and a Nanospray Flex Ion-Source
Data format Excel Table 1: 2D-SNO DIGE
Excel Table 2: SNOSID
Excel Table 3: SNO-SILAC
Excel Table 4: Gene ontology of redox modified proteins (pooled hits of 2D-DIGE, SNOSID and SNO-SILAC)
Excel Table 5: SNO-BIAM switch
Excel Table 6: Redox-BIAM switch
Experimental factors Cell treatment and harvest, protein extraction and labeling of redox sites, storage at -80°C, chromatography and MS analysis, MaxQuant software and Perseus software, gene ontology annotation
Experimental features nNOS+ (neuronal nitric oxide synthase, NOS1) and MOCK transduced SH-SY5Y neuroblastoma cells with rapamycin versus vehicle treatment (time course, 6 h and 24 h) or serum-free starvation for 24 h versus full-nutrient conditions
Data source location Frankfurt, Germany
Data accessibility Data is with this article