Subject area |
Redox Biology, Neuroscience, Proteomics |
More specific subject area |
Redox biology, Neurobiology, Aging, Protein homeostasis |
Type of data |
Spreadsheets |
How data was acquired |
Liquid chromatography / mass spectroscopy. Thermo Scientific LTQ Orbitrap XL or Thermo Scientific™ Q Exactive Plus equipped with an ultra-high performance liquid chromatography unit and a Nanospray Flex Ion-Source |
Data format |
Excel Table 1: 2D-SNO DIGE |
Excel Table 2: SNOSID |
Excel Table 3: SNO-SILAC |
Excel Table 4: Gene ontology of redox modified proteins (pooled hits of 2D-DIGE, SNOSID and SNO-SILAC) |
Excel Table 5: SNO-BIAM switch |
Excel Table 6: Redox-BIAM switch |
Experimental factors |
Cell treatment and harvest, protein extraction and labeling of redox sites, storage at -80°C, chromatography and MS analysis, MaxQuant software and Perseus software, gene ontology annotation |
Experimental features |
nNOS+ (neuronal nitric oxide synthase, NOS1) and MOCK transduced SH-SY5Y neuroblastoma cells with rapamycin versus vehicle treatment (time course, 6 h and 24 h) or serum-free starvation for 24 h versus full-nutrient conditions |
Data source location |
Frankfurt, Germany |
Data accessibility |
Data is with this article |