FIGURE 2.

Aged IP₃R2 KO mice display decreased NeuN⁺ neuron densities and increased S100β⁺ astrocyte densities. (A) Scheme of the prelimbic subregion of the medial prefrontal cortex (mPFC, blue) and respective layers I, II/III, and V. mPFC layer V (white dash line) corresponds to the area considered for cell counting. (B) Representative mPFC layer V confocal images of DAPI/NeuN labeling for WT and IP₃R2 KO mice (adult and aged). (C) Representative mPFC layer V confocal images of DAPI/S100β labeling for WT and IP₃R2 KO mice (adult and aged); (A–C) scale bars = 200 μm. (D,E) Densities of NeuN⁺ (D) and S100β⁺ (E) cells in mPFC layer V of WT and IP₃R2 KO mice (adult and aged; n = 8–15 images per group; two-way ANOVA, Sidak’s multiple comparisons test). WT (adults and aged) mice are represented in filled/unfilled black bars, whilst IP₃R2 KO (adults and aged) are represented in filled/unfilled red bars. Data plotted as mean ± SEM. ^#p < 0.05 and ^##p < 0.01 between genotypes and ^∗∗p < 0.01 between ages.