Figure 1.

T cell‐dependent activation induces higher antibody titres in Trim21‐deficient mice. Trim21 ^+/+ and ^–/– mice were immunized subcutaneously with 4‐hydroxy‐3‐nitrophenyl ovalbumin (NP‐OVA) in complete Freund’s adjuvant on day 0 and boosted with incomplete Freund’s adjuvant on day 14. Blood was collected for serological analysis at days 0, 5, 7, 14, 21 and at the end of the experiment at day 28 (Trim21 ^+/+ n = 9, Trim21 ^–/– n = 10). NP‐specific antibodies were measured by enzyme‐linked immunosorbent assay (ELISA) in sera. (a) Anti‐NP‐OVA immunoglobulin (Ig)M and total IgG titres. (b) Anti‐NP‐OVA IgG_1, IgG_2a, IgG_2b and IgG₃ subclass analysis. Data are presented as mean ± standard error of the mean (s.e.m.). *p < 0·05, **P < 0·01 (Mann–Whitney U‐test)