Figure 3.

T cell‐independent type II activation results in a stronger response in Trim21 ^–/– mice than T cell independent type I stimulation. Mice were immunized intraperitoneally with 4‐hydroxy‐3‐nitrophenyl‐lipopolysaccharide (NP‐LPS) or NP‐Ficoll in phosphate‐buffered saline (PBS) at day 0 and serum was collected at days 0, 5, 7 and 14. NP‐specific antibody levels were assessed by enzyme‐linked immunosorbent assay (ELISA). (a) Anti‐NP‐LPS immunoglobulin (Ig)M and IgG titres (Trim21 ^+/+ n = 9, Trim21 ^–/– n = 10). (b) Anti‐NP‐Ficoll IgM and IgG titres (Trim21 ^+/+ n = 9, Trim21 ^–/– n = 10) (data are presented as mean ± standard error of the mean (s.e.m.). *P < 0·05 (Mann–Whitney U‐test)