Figure 5.

Follicular B cells are expanded in unmanipulated Trim21‐deficient mice. The cellular composition of spleens of naive, unmanipulated mice was assessed by flow cytometry. (a) Frequency of CD19⁺ cells and (b) CD19⁺CD21⁺CD23⁺ follicular B cells, CD19⁺CD21⁺⁺CD23⁺ marginal zone B cells and CD19⁺CD21^–CD23^– T1 transitional B cells in mice aged 8–16 weeks (n = 17 per group, pooled data from four experiments). (c) Frequency of CD19⁺ cells and (d) CD19⁺CD21⁺CD23⁺ follicular B cells, CD19⁺CD21⁺⁺CD23⁺ marginal zone B cells and CD19⁺CD21^–CD23^– T1 transitional B cells in mice aged 52–58 weeks (Trim21 ^+/+ n = 25, Trim21 ^–/– n = 29, pooled data). Data are presented as mean ± standard error of the mean (s.e.m.). *P < 0·05, **P < 0·01 (Mann–Whitney U‐test)