Figure 4.

Identification of phosphoglycerate kinase 1 (PGK1) antigenicity in serum of Kawasaki disease (KD). (a) Results of Western blotting. Three positive results among 15 KD patients are displayed as lanes KD1, KD2, KD3. A 45‐kDa band was observed in all three cases. Two negative results were selected randomly among 12 negative results, and are displayed as lanes KD4, KD5. All FC and healthy control (HC) serum samples showed negative results. Ab: positive control (using anti‐PGK1 polyclonal antibodies instead of serum sample). (b) Immunoprecipitation of PGK1 protein with the sera from KD patients was performed. A 45‐kDa band can be clearly observed in the immune complexes. Lane M represents protein marker. (c) Validation of immune complexes contained rhPGK1 by Western blotting. Lanes 1 and 2 represent purified PGK1 serving as positive control.