Figure 2.

Frequencies of CD4⁺CD45RO⁺CD25⁺CD127^low regulatory T cells in peripheral blood mononuclear cells (PBMC) from healthy controls and patients with periodontal disease. PBMC were isolated from peripheral blood from patients with chronic periodontitis (CP, n = 15) or aggressive periodontitis (AP, n = 15) as well as periodontally healthy controls (HC, n = 13). Cells were surface‐stained and then permeabilized and stained intranuclearly for forkhead box protein 3 (FoxP3). Representative dot‐plots and cumulative data showing the frequencies of CD4⁺CD45RO⁺ T cells (a) and CD25⁺CD127^low cells within CD4⁺CD45RO⁺ T cells (b). (c) Representative histogram plots showing the expression levels of FoxP3 in CD4⁺CD25⁺CD127^low T cells. Each symbol represents an individual donor. Lines show the mean with standard error of the mean (s.e.m.). Log‐transformed data were analysed by one‐way analysis of variance with Bonferroni’s multiple comparison test.