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. 2018 Oct 21;194(3):380–390. doi: 10.1111/cei.13205

Figure 4.

Figure 4

Frequencies of cytokine producing CD4+ T cells in PBMC from healthy controls and patients with periodontal disease. Peripheral blood mononuclear cells (PBMC) were isolated from peripheral blood from patients with chronic periodontitis (CP, n = 15) or aggressive periodontitis (AP, n = 15), as well as periodontally healthy controls (HC, n = 13). Cells were stimulated ex vivo with phorbol myristate acetate (PMA) and ionomycin for 3 h prior to intracellular cytokine staining. (a) Representative gating strategy and (b) cumulative data showing the frequencies for the interleukin (IL)‐17‐, interferon (IFN)‐γ‐, tumour necrosis factor (TNF)‐α‐ and IL‐10‐expressing cells within CD4+ T cells as determined based on fluorescence‐minus‐one (FMO) control. Each symbol represents an individual donor. Lines show the mean with standard error of the mean (s.e.m.). Log‐transformed data were analysed by one‐way analysis of variance with Bonferroni’s multiple comparison test.